Abstract
Summary
Technics are presented for obtaining monolayer cultures of human fetal cells from the chorionic villi of mature placentas. Mixed cultures of epithelioid and spindle cells are obtained with these methods. The fetal origin of the cells in culture is confirmed with studies of the sex chromatin pattern of the nuclei and by examination of histological sections of the trypsinized tissue for the presence of decidua. Detection of chorionic gonadotrophin in the medium of 2 cultures of cells from mature placentas is reported.
The investigators are indebted to Dr. Herbert R. Morgan for consultation and advice during these studies and to Virginia Eldridge and Eleanor Dudley for technical assistance.
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