Abstract
Summary
Sprague-Dawley-Rolfsmeyer rats were ovariectomized in estrus, then administered progesterone (P) at rate of 1.5 mg/day for 5 days (pretrauma period). Endometrium of one uterine horn of each animal was scratched throughout its entire length by sharp needle. During post-trauma period, days 6 to 9, P at levels of 1, 2, 3, and 6 mg was injected daily. On day 10 each uterus was separated into control and traumatized horn, weighed and processed for DNA determination. The traumatized horns showed a graded increase in wet weight, DFFT, and DNA/100 g B.W. over control horns when 1, 2, and 3 mg/day of P were administered during post-trauma period. Six mg/day did not increase reaction further. Considerable variation in reaction occurred in each group requiring a considerable number of animals for use as assay method.
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