Abstract
Summary
A study was made of factors involved in modifying the semi-permeable nature of the cell membrane of Ehrlich ascites tumor cells. It was found that a minimum concentration of 0.1% nigrosin was necessary for accurate differential quantitation of modified and unmodified tumor cells. The cells were not visibly affected by hypotonic saline solutions as low as 3.0 mg NaCl/ml. Lower concentrations resulted in changes in membrane permeability, and lysis occurred at concentrations of 1.0 mg/ml, or less. Not all cells of a given population were affected similarly by experimental hypotonic shock.
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