Abstract
Summary
The effects of centrifugation at 20,000 × g for 2 hours on lipid distribution of various human sera have been studied. In general, when neutral fat was below 176 mg%, very little, if any, cholesterol or phospholipids rose to the surface. When neutral fat content was markedly elevated, as much as 90% of both cholesterol and neutral fat and 50% of phospholipids underwent flotation. However, subjects with similar degrees of marked hyperlipemia appeared to separate into 2 major groups represented by moderate and marked degrees of flotation of neutral fat, the latter group being composed primarily of subjects with familial hyperlipemia. An inverse correlation was found between whole serum neutral fat content and percentage of total cholesterol remaining in the subnatant serum. Variations from these general patterns and possible clinical interpretations are being studied.
We gratefully acknowledge a grant from the Richmond Area Heart Assn. in support of our work on lipid metabolism. Our thanks are due Dr. William Burton and Dr. Morton Bender as well as many practicing physicians in Richmond for collecting blood samples used in this study.
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