Abstract
Summary
1. Both compound 48/80 and stilbamidine interact directly with strandin at 6°C to form turbidimetric interaction products. 2. The fluorescence of stilbamidine is quenched by strandin, the effect being a function of the strandin concentration. 3. Strandin exerts a marked effect upon the UV spectrum of stilbamidine, optical density rising above the theoretical below 280 mμ, and falling well below the theoretical curve above 280 mμ 4. Compound 48/80 causes a reduction in turbidity of the strandin-lysozyme and the strandin-protamine systems. In the case of the lysozyme system the reaction was markedly reversed by readdition of strandin. This was not the case with the protamine system. With stilbamidine similar results were obtained with both lysozyme-strandin and poly-l-lysine-strandin. In the case of protaminestrandin, stilbamidine caused an increase in turbidity of the system. 5. Both compound 48/80 and stilbamidine suppress the metachromasy of the toluidine blue O-strandin system. 6. Strandin was found effectively to suppress the interaction between chondroitin sulfate and stilbamidine, and the interaction between Germanin and stilbamidine. 7. A mechanism involving a reversible equilibrium between these compounds and strandin has been proposed to explain the results obtained.
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