Partial Replacement of Serum and Embryo Extract Requirements for Growth of Avian Cell Cultures. ∗

Abstract

Summary

Salmine, pyruvate, insulin and lactalysate or its derivatives have been shown to replace partially requirements of avian cell cultures for serum and embryo extract. In addition to these factors, however, serum was required for growth comparable to that attained in the basal medium containing both serum and embryo extract. A function of lactalysate and embryo extract was inhibition of a toxic effect of serum. The stimulatory and“anti-toxic” effects of lactalysate were confined largely to the non-dialyzable portion. Nevertheless, the activity of acid hydrolysates of the lactalysate residue demonstrated that these properties were not dependent on a proteose or peptone.