Abstract
Summary
The use of a feeder layer of x-irradiated or non-irradiated primary human amnion monolayers in cultivation of leukemic and normal human bone marrow aspirates results in establishment of primary, human fibroblastic cell cultures. Comparison of normal and leukemic cultures failed to reveal differences in morphology, type of degeneration, rate of metabolism or viral susceptibility. A cytopathogenic agent was not recovered from leukemic aspirates and use of the fluorescent antibody technic failed to reveal a specific antigen capable of reacting with leukemic sera in leukemic bone marrow cell cultures. Cultures of cells from both leukemic and normal bone marrow supported multiplication of polio, herpes simplex and measles virus. Normal marrow cell cultures supported multiplication of Sindbis and influenza A virus, but not of an egg adapted strain of mumps virus. The reproducible cultivation of cells obtained from human bone marrow provides an accessible source of primary human fibroblastic cell culture that may prove useful in both microbiological and hematological experimentation.
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