Abstract
Summary
Isolation of mast cells from peritoneal washings of the rat in either a sucrose or Ficoll density gradient gave the same activity of a succinic dehydrogenase system in the cells. Disruption of isolated cells by washing them with a Versene-phosphate buffer (pH 7.7) greatly elevated the enzyme activity. Cold stress or subcutaneous injection of ACTH, E. coli endotoxin, or compound 48/80, increased activity per unit protein-nitrogen significantly, and independently of gross cell disruption, since all cells were disrupted for measurement. No effects on activity of age from 30 to 120 days, or of subcutaneous injections of histamine or serotonin were observed.
The authors are grateful to Dr. A. C. Parekh and Dr. L. J. Greenberg for suggestions and technical help.
Get full access to this article
View all access options for this article.