Abstract
Summary
A method of assaying the potency of sera prepared against the mouse mammary tumor agent using fluorescent dye labeled antisera is described. Better correlation between this method and serum neutralization tests, with transplanted Z tumor in Z strain mice, were found than in routine serologic testing. Agglutination, precipitin, and complement-fixation tests were found to be completely unreliable when testing anti-Z tumor sera. Use of Lissamine-Rhodamine RB-200 and isothiocyanate as described by Smith et al.(8) eliminated most nonspecific fluorescence and gave a better analysis of the reactive sites. Similar results were found using antisera which had been adsorbed with normal tissue and the globulins fractionated by ammonium sulfate and then conjugated with isothiocyanate. Little or no reaction was observed when either of these materials was tested against normal tissues or tumors not related to the inbred strain Z.
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