Biochemical Changes and Incorporation of P 32 in Separated Elements of the Hyperplastic Lesion of Fowlpox

Abstract

Summary

Normal control and fowlpox-infected chick scalp were separated by trypsin into connective tissue and epithelium. By appropriate technics the infected epithelial tissue was separated into surface epithelium and follicles. Comparison was made between normal epithelium and connective tissue and between normal and infected tissues with respect to certain lipids and incorporation of P³² into phospholipid. Distinct differences in phospholipid and total, free, and esterified cholesterol were found between normal epithelium and connective tissue. The above components were increased 2- to 3-fold at some interval of time in both infected epithelium and connective tissue. The specific activities of phospholipid P from all tissues were approximate at 3 days but that of normal epithelium and connective tissue fell approximately 50% at 5 and 7 days. In contrast the incorporation of P³² in infected tissues was 1.5 to 1.7 times that of normal controls.