Abstract
Summary
Bovine kidney culture cells were found to be resistant to high concentrations of poisons of the glycolytic cycle, the citric acid cycle, and the cytochrome oxidation chain, as measured by respiration and cell loosening from the glass. Infection with FMDV had no influence on the results, and it may be that respiration of the cells is more dependent on other metabolic cycles. Iodo-acetate and fluoride, present in concentrations which caused large decreases in glycolysis, decreased virus production more than any other poisons. Malonate had an insignificant effect on virus production, while the larger effect of fluoroacetate was probably due to its action in removing viral precursors. The fact that maintenance of infected cells in an anaerobic environment did not affect virus production was a further indication that glycolytic sequences of the host cell are more essential to viral replication than oxidative sequences.
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