Abstract
Summary
1. An improved method for demonstration of lactic dehydrogenase after starch gel electrophoresis is described. This method employs a more sensitive tetrazolium and phenazine methosulfate. 2. Rat kidney contained 5 lactic dehydrogenase fractions which varied in cofactor requirements. DPN diaphorase was not demonstrable in any of these fractions, when reduced DPN was employed as the substrate, but was demonstrated in 2 additional bands. 3. The possibility is discussed that at least certain of the lactic dehydrogenase fractions from rat kidney exhibit electrophoretic heterogenity because of their association with various components of the respiratory system.
We are deeply indebted to Mrs. Julia Ternak for technical assistance.
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