Abstract
It has been reported from this laboratory that the topical application of iodoacetamide (IAA) or N-ethyl maleimide (NEM) solutions to the mucosa of canine gastric pouches will evoke a flow of mucinous secretion for many hours (1). This material had never been recognized before, and because its physiological significance is still unknown, it was designated “para-mucus” (2). Exploratory experiments on the pepsin content of paramucus indicated that the zymogen is present in appreciable quantities, and that it is probably a significant component in chemical characterization of this mucinous secretion. This report presents a systematic study of the variations in pepsinogen content of para-mucus, using specimens collected fractionally every 30 minutes for 6 hours following topical application of IAA solution to the gastric mucosa in dogs with a Heidenhain pouch and antrectomy. Several other characteristics of this material are presented here.
Material and method. Four Heidenhain pouch dogs, provided with an antrectomy, were used in these experiments. The antral resection was performed to insure the virtually complete absence of HC1 secretion in the fasting state, in accordance with previous experience in this laboratory (3) . Each dog was subjected to the follolwing 2 types of experiment: A) Histamine stimulation : Dogs were starved for at least 18 hours before experiment, and unstimulated (basal) secretion was collected for 2 hours, at 60-minute intervals. Following this, histamine was administered subcutaneously (0.1 mg of histamine basekg body weight) and the acid secretion was collected for two 60-minute intervals thereafter. Such experiments were done to be sure that the acid-secretory behavior of the dogs was normal. B) Topical application of 1AA:1AA in water solution, at a concentration of 3mM (55.5 mg 100 ml) was applied topically for 30 minutes, and secretion was collected every 30 minutes for 6 hours (1 2 experiments). The technics were those described previously (1).
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