Abstract
Summary
It is shown that certain antibodies engendered in “J-negative” (or A-negative) rabbits by immunizing with human red cells of type A will selectively lyse red cells of J-positive cattle. Some of the “J” reagents so produced have proved to be either equal to or superior to the better J reagents obtained from J-negative cattle in their ability to promote lysis of the more weakly reactive intergrades of J-positive blood. The key to recognition of these specific cross reactions was substitution of guinea pig complement for rabbit complement. The method of classifying J-positive and J-negative rabbits is described and data are presented on distribution of the J types in 110 rabbits.
We are indebted to the Sacramento Medical Foundation Blood Bank for human blood used in this study.
Get full access to this article
View all access options for this article.