Concentrated Culture of Gonococci in Clear Liquid Medium. ∗

Abstract

Discussion and summary

The results first demonstrate the applicability and usefulness of an agar/liquid flask system to obtain gonococcal growth several times more dense than normal in a representative liquid medium. Counts as high as 5 × 10⁹ cells per ml and turbidity similar to that normally seen with staphylococci were attained.

Second and perhaps more significant, the results illustrate a principle by which the enrichment materials normally incorporated into gonococcal media can be separated from the immediate growth environment. Under the conditions existing in a biphasic flask, even a clear water extract of the complete medium supported luxuriant growth of gonococci. Such medium simplification in principle should be applicable to other fastidious organisms.