Abstract
Summary
Livers of mice infected with mouse hepatitis virus (MHV 1) were examined by conventional staining procedures and by electron and fluorescence microscopy using acridine orange fluorochrome and fluorescein-tagged antibody. Preparations stained with acridine orange showed abnormal accumulations of RNA-staining material in cytoplasm of hepatic cells. Inclusion bodies of similar size, shape, and location were stained selectively with fluorescein-tagged antibody. Observations by electron microscopy showed virus particles in cytoplasm which measured 90 ± 20 mμ in diameter.
The authors are indebted to Dr. R. H. Rigdon and his staff for invaluable technical assistance.
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