Abstract
Summary
1) Effects of sodium fluoride, sodium azide, sodium arsenite and urethan on growth, glucose utilization, protein utilization and mitosis in strain L cells were studied. 2) Cell populations in control cultures averaged 3.17 million/ml after 5 days; had utilized an average of 0.99 mg of glucose and 4.0 mg of protein/ml of medium. 3) Fluoride (10-3 M), azide (10-4 M) and arsenite (10-5 M) decreased cell numbers by 24, 63 and 81%, respectively. Although urethan (10-1 M) killed the cells, a concentration of 10-2 M had little effect. 4) Fluoride (10-2 M), arsenite (10-4 M) and urethan (10-3 M) inhibited glucose uptake by 28, 23 and 8%, respectively. Azide (10-3 M) produced 10% acceleration of glucose uptake. 5) Azide (10-3 M) and fluoride (10-3 M) inhibited protein uptake by 92 and 39%, respectively. Arsenite and urethan accelerated protein uptake in all concentrations. 6) The mitotic index of untreated cells was 10.5. All inhibitors except azide produced a lowered index. 7) In control cultures, 4% of cells were multinucleate. Azide (10-4 M) increased these forms to 17%.
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