Abstract
Summary
1. For sustained growth of mouse fibroblast cells (strain L) in suspended culture in Eagle's medium, periodic addition of arginine is essential. This requirement can be provided by arginine alone or by equimolar amounts of citrulline and aspartic acid.
2. These cells contain enzymes which catalyze the breakdown of arginine to ornithine and 2 equivalents of ammonia.
3. The requirement for periodic addition of arginine or its precursors is explained by continuous rapid breakdown of arginine observed during growth.
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