Abstract
Summary
1. Various parameters involved in assay and growth of HA1 virus in tissue culture have been examined. Variation in occurrence of cytopathic changes in different cell systems led to development of a new plaque assay technic utilizing hemadsorption properties of the virus. HA1 is adsorbed to cell monolayers rapidly from small volumes (0.1 ml) so that 1 hour adsorption is adequate for routine plaque work. Thermal inactivation curve showed a halflife at 37°C of 10 hr. Presence of antibody to HA1 in human and calf serum, and in serum from guinea pigs inoculated intranasally confirms work by others and emphasizes the need to pretest all sera used in work with this virus. Growth curves showed that at end of logarithmic increase, concentration of virus in the supernatant fluid was less than 0.1% that in cells. An average of approximately 50 pfu was produced/cell.
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