Abstract
Summary
A method is described for measurement of glucose-6-phosphate in tissue extracts by use of glucose-6-phosphate dehydrogenase. Interference resulting from contamination of this enzyme with glutathione reductase was abolished by addition of a mixture of HgCl2 and glutathione. Hexokinase, another contaminating enzyme which interfered with the determination, was inhibited by removing Mg ion. Phosphoglucomutase and phosphoglucoseisomerase were not present in significant amounts.
The authors are grateful to Dr. Carl F. Cori for encouragement and advice, and thank Drs. Robert K. Crane for a preparation of fructose-6-phosphate and David H. Brown for glucose-1,6-diphosphate.
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