Abstract
Summary
Primary chick embryo and maturing cockerel lung cultures were found to grow in a test medium containing dialyzed chick embryo extract, bovine serum, and a variety of small molecular constituents. The avian cultures required the 13 amino acids usually found to be essential for mammalian cells. They also were markedly stimulated by glycine or L-serine and by small quantities of L-alanine. Folinic acid and higher levels of folic acid partially replaced the glycine requirement for optimal growth. Pyruvate, oxalacetate, or alpha-ketoglutarate, replaced the requirement for L-alanine. Pyridoxal phosphate partially replaced the L-alanine.
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