Abstract
Summary
Radioiron assay of erythropoietin has been analyzed and for purposes of inter-lab comparison of erythropoietic activity. the response to 5 μmoles of cobalt, given by subcutaneous injections, is suggested to represent 1 unit of activity. A house standard of partially purified sheep erythropoietin, having a potency of 1 unit/mg, has been used to establish a dose-response curve up to 22.4 units rat. In this range the curve shows an increasing slope to 11.3 units/rat. On the basis of this assay, the potency of several fractions of sheep erythropoietin has been given.
The technical assistance of M. L. Wang and L. Martino and helpful suggestions of J. D. Fisher are gratefully acknowledged.
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