Essential Growth Factor in Serum Dialysate for Chick Skeletal Muscle Fibroblasts. ∗

Abstract

Summary

Chick skeletal muscle fibroblasts in monolayer cultures grow actively in homologous undialyzed serum supplemented with Medium 199. Dialysis of chicken serum removes a factor essential for growth and maintenance of these cells. The deficiency syndrome in cultures with dialyzed serum can be corrected by chicken serum dialysate, but not by inositol, cholesterol, vit. B₁₂, spleen nucleoprotein fraction, or by a more complex synthetic nutrient, NCTC 109. Proteosepeptone at 0.1% may be substituted for serum dialysate to restore full activity of whole serum.