Abstract
Summary
Serial cultivation of cells obtained from the human pituitary is described. Somatotropin, corticotropin, and gonadotropin have been detected in these cultures. Two of the cell lines have produced gonadotropin, both FSH and ICSH, when in suspension culture, and one, S4374-M, has been frozen and recultivated in both stationary- and suspension types of culture with media containing 10% of human, equine or bovine serum, with resumption of production of hormone. The gonadotropin-producing cells have the microscopical appearance of a chromophobe type of pituitary cell and have a characteristic appearance when stained by the iron-PAS method. These cells are heteroploid with a chromosome count in narrow range with modality at 79.
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