Abstract
Summary
A method is described for determining penicillin in poliomyelitis vaccine. This method consists of acidifying the vaccine, extracting the penicillin into diethyl ether, removal of ether by vacuum distillation and transfer of penicillin into a buffer. The penicillin is determined by standard paper chromatographic and bioautographic technics. This method is capable of detecting 0.001 unit of penicillin/ml finished vaccine.
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