Abstract
Summary
An agglutination method for detecting antibodies against blood lymphocytes is described. By this method non-specific clumping of polymorphonuclear leucocytes and/or monocytes usually encountered in leucoagglutination tests is avoided. The humoral response following homografting in chickens was reinvestigated using this lymphoagglutination reaction. In 82 combinations of normal sera or sera from autografted chickens, and lymphocytes, lymphoagglutination was found in only 5 instances. On the other hand, 103 cases of positive agglutination were obtained in tests with 107 combinations of immune sera and normal lymphocytes. These immune sera, including one with titer of 1:1024, were from animals previously injected with pooled homologous spleens. Sera from animals injected with splenic cells or grafted with skin from one donor, also contained lymphoagglutinins against lymphocytes of spleen or skin donor chicken. These lymphoagglutinins also reacted in some instances with lymphocytes of chickens other than spleen or skin donor.
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