Abstract
Summary
Apparent free glycine and apparent hippuric acid in urine have been determined by microbiological analysis after fractionating it by a 4-cell, 7-transfer countercurrent distribution between n-butanol and 5% hydrochloric acid. The effectiveness of this procedure was demonstrated by separating hippuric acid from free glycine in known mixtures and by recovering known amounts of these metabolites added to urine before subjecting it to the fractionation procedure. The apparent concentrations of free glycine and hippuric acid in normal pooled urine were 3.1 and 4.6 μ/ml, respectively.
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