Abstract
Summary
A cloned wild-type population (strain D98S) derived from human bone marrow cells, which is inhibited by 8-azaguanine (AG) in concentrations in excess of 0.1 μg/ml, contains approximately 1 to 2% cells resistant to AG in 100-fold higher concentrations. A single-step mutational process (in broad meaning of the word) appears to be involved. The AG-resistant lines are stable upon prolonged subculture either in presence or absence of drug. Mutation rate from sensitivity to resistance is of the order of 5 × 10-4cell/generation. AG resistance is a useful genetic marker, since at selective AG concentrations (a wide plateau at 1-10 μg/ml) colony formation by resistant cells is not impaired and sensitive cells are destroyed. A method for protein determination is described, based on measurement of eluted bromphenol blue from HgCl2-fixed, glass-attached cells.
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