Abstract
Summary
1) RNA-containing fractions have been prepared from crude tissue culture virus suspensions of 6 varieties of enterovirus by the method of Gierer and Schramm. Each RNA preparation has produced plaques on human cell monolayers. The plaque-forming agent is completely inactivated by RNAase but not by DNAase. RNA is, therefore, an essential component for the infectivity of the preparation. 2) The plaques formed by RNA fraction contain intact virus from which RNA is prepared. The RNA directs the cell to replicate not only the specific RNA but also a new highly specific protein needed for synthesis of intact original virus.
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