Abstract
Summary
A large decrease in cytoplasmic pyroninophilia and thioninophilia resulted from digestion of formalin-fixed monkey kidney tissue culture with either chymotrypsin or ribonuclease. This pair of enzymes decreased cytoplasmic pyroninophilia and thioninophilia more than either enzyme alone. Digestion of similar cultures with desoxyribonuclease gave a large decrease in nuclear pyroninophilia and thioninophilia. The effects of these 3 enzymes on pyroninophilia of these cells were more clearly shown when a purified pyronin was used.
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