Virologic Use of Monkey Kidney Cells Preserved by Freezing. †

Abstract

Summary

Cells of freshly trypsinized monkey kidney and of primary cultures of monkey kidney were preserved by slow freezing in 5% glycerol medium and storage at −75°C. Cultures of thawed cells yielded monolayers that could be directly utilized for virologic work. In these, the cytologic response of plaque morphology due to polioviruses, ECHO, Cox-sackie, measles, herpes simplex, influenza, CA, HA–1, JH, 2060, and adenovirus, as well as assays by tube titration or plaque counts, were equivalent to those produced in unfrozen cells. Moreover, isolations of ECHO 9, Cox-sackie A–9, B–5, and polioviruses, from stools or cerebrospinal fluids were made with equal facility in either type of culture.