Abstract
Summary
Rate of formation and release of poliovirus Type 1 were studied at 32° and 37°C in FL cell cultures. Virus accumulated almost as rapidly and reached practically the same intracellular levels at 32°C as at 37°C. However, release of virus was much slower at 32° than at 37°C and as a result, at 32°C the intracellular concentration of virus remained at or near peak levels for 4 to 6 hours. From these observations, highly potent virus for use in immunological studies was prepared by mechanical disruption of infected cells maintained at room temperature (26–32°C) for 18–20 hours.
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