Abstract
Summary
Extracts of hog stomach mucosa were fractionated on cellulose-cation exchanger, carboxymethylcellulose. Highest activity for increasing the combining of Vit. B12 with receptor substances of tissue was eluted between pH 4.2 and pH 4.4. Highest activity for intrinsic factor by the Schilling technic was eluted between pH 4.4 and pH 4.7. An approximate pK (or isoelectric point) of pH 4.5 was indicated for the material with intrinsic factor activity by the Schilling assay. These fractions contained 2 to 3% sialic acid.
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