Loss of Hemagglutinin on Passage of Vaccinia Virus in L Cells. ∗ †

Abstract

Summary

Cell-free ascitic fluids obtained from mice bearing Ehrlich tumor contain an inhibitor which prevents vaccinia virus hemagglutination. Serial passage of the virus in tissue cultures of L cells in presence of the inhibitor favors appearance of virus which has lost ability to produce hemagglutinin. Serial passage of the virus under comparable conditions in the absence of inhibitor did not influence hemagglutinin-producing activity of the virus. These results support the assumption that the loss of hemagglutinin-producing ability by vaccinia virus, on passage through the Ehrlich tumor, results from action of the inhibitor.