Abstract
Summary
A method of bioassay for small amounts of antidiuretic hormone in plasma of a dog is presented. The method utilizes rats rendered unconscious with ethanol, under constant water load, and with intravenous injections of all standards and unknowns. Change in specific gravity of rat urine expressed as antidiuretic index is used as criterion of potency. This method appears to be the most sensitive bioassay for ADH available in that small dose changes of reference vasopressin, or endogenous circulating ADH, can be differentiated.
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