Abstract
Summary
1) Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating CRF. An in vivo assay was used to assess CRF activity. 2) The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.
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