Abstract
Summary
The Gledhill strain of mouse hepatitis virus (MHV 1) was passaged through 10 successive tissue cultures of newborn mouse kidney explants. As MHV 1 did not cause reproducible cytopathogenic effects, infectivity tests with newborn mice were used to detect propagation of this virus in serially passaged cultures. Observations suggest that virus was adsorbed by the proliferating explants and that cell-free culture fluids were infective. Total time of virus growth far exceeded decay rate of the virus incubated without tissue. After the tenth passage, the virus had been diluted 1012-fold which exceeded the extinction point of the original inoculum.
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