Abstract
Summary
A method for estimating insulin using pooled mouse hemidiaphragms has been developed. A linear log dose-response was obtained between 30–3000 micro-units of insulin/ml. Basal glucose uptake of mouse diaphragm tissue was 2 1/2 to 4 times that of rat diaphragm tissue. Basal uptake was significantly affected by pH of medium, glucose concentration, and diaphragm size. A progressive increase in magnesium or potassium concentration of the medium suppressed the basal glucose uptake.
Get full access to this article
View all access options for this article.