Variations in the amount of transformed atoxyl (trypanotoxyl) produced by varying the strength of atoxyl incubated with blood

Abstract

A 10 per cent. solution of atoxyl in blood incubated for 1 to 3 hours at 37° C. is more than 10 times as toxic for Trypanosoma brucei as a 1 per cent. solution in blood similarly incubated. To avoid misleading results due to the transforming action of red blood corpuscles upon unaltered atoxyl continuing after the dilutions are made, immediately after incubation all red blood corpuscles should be removed by centrifugalization from the fluid containing the transformed atoxyl.