Pancreatic transplantations in the spleen

Abstract

The influence of pancreatic transplants in the spleen on the prevention of diabetes was studied. The spleen was selected because it has been shown that transplanted bits of tissue are especially well nourished in the pulp of this organ. In nine animals, bits of the pancreas of various size were buried in the pulp of the spleen. The method used by Payr in transplanting thyroid tissue was followed. In four animals (2 dogs and 2 cats) pancreatic cells were demonstrable in the spleen. The interval between the operation and the death of the animal varied from 18 hours to 13 days. In five animals (all dogs) no pancreatic remains were found. Autolysis was rapid. In one experiment all the pancreatic cells had disappeared 21 hours after the transplantation. The bit of tissue found 13 days after the transplant measured only 1 mm. by 0.1 mm. It consisted of normal appearing acini surrounded by connective tissue. No islands of Langerhans were demonstrable.

In one dog a large pancreatic graft was placed in the spleen with the blood supply preserved by means of a mesenteric stalk. Three weeks later the original blood supply was cut off and all the pancreatic tissue except the graft extirpated. Diabetes did not develop, but the tolerance for glucose fell within a few weeks to a low point. At the autopsy 187 days after the second operation a large abscess was found in the lower part of the spleen. Projecting into the spleen from the wall of the abscess was a coneshaped mass of fibrous tissue. In this were the remains of the pancreatic transplant measuring less than I cm. in length. The pancreatic tissue consisted of acini separated by connective tissue.