Abstract
In collaboration with Dr. F. P. Gay, a study has been made of the glycogen in the livers of 22 rabbits, comparing the chemically determined amount with the histological appearance with a view to finding the value of the latter for comparing small differences in glycogen content. Pflüger's method was employed as far as the conversion of glycogen to glucose and for the quantitative estimation of the latter Bertrand's method was used. For the histological picture, Best's carmine method and Langerhansmodification of Ehrlich's iodine method were employed.
The following table gives the comparative results. The chemical factors are reduced to a common denominator, viz., the amount of copper which is reduced by 100 gm. of liver, and arranged from the highest to lowest amounts. The histological results are placed in a parallel column. While in the main there is faily close correlation, yet there are two striking discrepancies (No. 23 and No. 24).
This study presents, so far as we can determine, the first attempt to correlate chemical and microchemical findings with a view to utilizing the latter for comparing slight differences of glycogen in the liver. The results do not warrant the assumption of parallel results between the chemical and microchemical results; hence the histological method is not available for accurate comparative work.
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