Abstract
Several dogs completely depancreatized by Hedon's method and eliminating glucose and nitrogen in Minkowski's ratio were treated by intravenous injection of pancreatic extract prepared by Knowlton and Starling's method. 1 The urines collected in twenty-four hour periods exhibited an increase in the D: N ratio on the days immediately following. When the urine was collected in short periods after injection, a marked fall in the sugar output was witnessed lasting from four to ten hours but this was followed by a compensating rise which, in some instances, augmented the total for the twenty-four hour period, in others raised it only to the previous level.
Much greater effects were obtained with a double extract of dog's pancreas and duodenal mucosa. The following experiment (No. II) is typical. The effect, however, cannot be ascribed to the organic extract from either the pancreas or the duodenum for the same Ringer's solution in which the tissues were extracted when made alkaline to about the same extent with Na2CO3 gave an identical effect.
The similarity in these two experiments on the same dog indicates that it is not a hormone which is responsible for the reduction in the sugar, and the increase in the percentage of sugar in the blood indicates that the reduced sugar elimination is in reality due to a change in the permeability of the kidney, as Wohlgemuth 2 has found it after ligation of the pancreatic ducts.
That there is no effect on the combustion of sugar attending the reduced elimination of that substance is proved by the following experiment on another dog place in the respiration calorimeter.
Already in the first hour's urine, including the period of injection, a marked decline in the sugar elimination is seen, showing that the typical effect on the excretion of sugar would have been produced, if glucose had not been fed. b'ith 20 grams of glucose available, however, none, or an extremely small quantity at the most, was burned.
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