Five metal compounds, zinc chloride (ZnCl2), mercury chloride (HgCl2), chromium chloride (CrCl3) hexahydrate, cadmium chloride (CdCl2) hemidihydrate, and nickel chloride (NiCl2) hexahydrate, were selected and tested for potential modifying influence on renal tumorigenesis. Six groups, each consisting of 15 male F344 rats, were given N-ethyl-N-hydroxyethylnitrosamine (EHEN) at a concentration of 500 ppm in their drinking water for the first 2 weeks as the initiation of carcinogenesis. Thereafter, the rats were treated orally for 25 weeks with ZnCl2, HgCl2, CrCl3, CdCl2, and NiCl2, respectively, at concentrations of 450, 40, 600, 100, and 600 ppm in the drinking water. The control group was given unsupplemented distilled water (DW) after EHEN initiation. Renal neoplastic lesions were classified histologically into dysplastic foci (DF) and renal cell tumors (RCT). As a result, statistically significant increases were found in the mean numbers of DF per cm2 in rats treated with the five metal compounds. On the other hand, the incidence of RCT was significantly higher only in rats treated with NiCl2. It is concluded that NiCl2 exerted a potential for promotion of renal tumorigenesis under the conditions of this study.
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