Measurement of Viability and Aryl Hydrocarbon Hydroxylase Activity in Cryopreserved Baboon Peripheral Blood Lymphocytes and Pulmonary Alveolar Macrophages

Abstract

Pulmonary alveolar macrophages (PAMs) and peripheral blood lymphocytes obtained from healthy nonsmoking baboons were evaluated for the ability to retain viability and aryl hydrocarbon hydroxylase (AHH) activity following cryopreservation for 2, 4, 6, 8, and 12 week intervals. Viability was measured (by trypan blue dye exclusion) in lymphocytes following 2, 4, 6, 8, and 12 weeks of cryopreservation. No significant decrease in viability was observed (P > 0.05 for all comparisons; nonpaired 2-tailed, t-test; n = 22), with lymphocytes retaining 88% of their original viability following 12 weeks of cryopreservation. Similarly, a slight, but insignificant decrease in PAM viability was observed following cryopreservation for 2, 4, 6, and 8 week intervals (P > 0.05 in all instances), with PAMs retaining 93.5% of the original 0 time viability. AHH activity was also evaluated in PAMs and lymphocytes following cryopreservation. Lymphocyte noninduced and BA-induced AHH activities were slightly but not significantly decreased after 2, 4, 6, 8, and 12 week cryopreservation intervals (P > 0.05 for all comparisons). Similarly, AHH activity in cultured PAMs was not significantly decreased following cryopreservation for 2, 4, 6, and 8 week intervals (P > 0.05 for all noninduced and BA-induced AHH levels assayed at the various time intervals; n = 8). Even following cryopreservation periods, for 8 or 12 week intervals, PAMs and lymphocytes retained 91% and 90%, respectively, of the original BA-induced AHH activity. These data demonstrate that baboon PAMs and lymphocytes are capable of undergoing cryopreservation while maintaining viability and AHH activity.

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