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Abstract

Induction of sperm abnormality in mice and hamster and reproductive toxicity in mice was examined following chronic exposures (ad libitum) to N-chloropiperidine (NCP). The results showed that significant levels of abnormal sperm in the NCP treated group were observed after five weeks of exposure. The time of the appearance of NCP-induced anomalous sperm suggested that the compound did not affect late spermatocytes and spermiogenesis. Reproductive toxicological analysis based on moles, implantations, and offspring per pregnancy revealed that while the toxic effect of ethylmethane sulfonate was evident within the first week of treatment, NCP-induced toxicity was observed only after five weeks of treatment. The data further showed that in matings where both parents were exposed to NCP, the frequencies of moles, implantations, and offspring per pregnancy were fewer than those observed in single parent exposure.

References

1. Bempong, M.A. and Scully, Jr., F.E. (1980). Mutagenic activity of N-chloropiperidine. J. Environ. Pathol. Toxiwl. 4(1), 345–354.

2. Bempong, M.A. and Scully, Jr., F.E. (1980). In vitro cytological effects of N-chloropiperidine: Induction of mitotic anomalies in Chinese hamster ovary cells. In “Water Chlorination: Environmental Impact and Health Effects,” vol. 3, R. L. Jolley , W. A. Brungs , and R. B. Cumming , Eds. Ann Arbor, MI, Ann Arbor Science Publishers Inc., pp. 817–825.

3. Bempong, M.A. , and Scully, Jr., F.E. (1982). Mutagenicity and clastogenicity of N-chloropiperidine. J. Environ. Pathol. Toxiwl. and Oncology 5(1), 473–483.

4. Bempong, M.A. and Scully, Jr., F.E. (1981). In vitro evaluation of N-chloropiperidine for toxic and mutagenic effects. J. Basic Appl. Sci. 39(1), 78–84.

5. Scully, F.E. Jr. , and Davis, R.C. (1978). Superoxide in organic synthesis a new mild method for the oxidation of mines to carbonyls via N-chloramines. J. Org. Chcm. 43, 1467

6. Bruce, W.R., Furrer, R. and Wyrobek, A.J. Abnormalities in the shape of murine sperm after acute testicular x-irradiation. 23, 381-386.

7. Soares, E.R. , Sheridan, W. , Haseman. J.K. and Segall, M. (1979). Increased frequences of aberrant sperm as indicators of mutagenic damage in mice. Mutat. Res. 64, 27–35.

8. Burgoyne, P.S. (1975). Sperm phenotype and its relationship to somatic and germline genotypes: A study using mouse aggregation chimeras. Develop. Biol. 44, 63–76.

9. Wyrobek, A.Y. and Bruce, W.R. (1975). Chemical induction of sperm abnormalities in mice. Proc. Natl. Acad. Sci. 72, 425–4429.

10.

10. Bempong, M.A. and Hall, E.V. (1981). Murine seminal cytology following oral administration of subacute doses of 1,3-diphenylguanidine. Presented at the Ninth Annual Biomedical Symposium, Albuquerque, April 3-6.

11.

11. Krzanowska, H. (1972). The influence of age on the proportion abnormal spermatozoa and on fertilization rate in inbred and F1 hybrid male mice. Acta. Biol. Cracov. Ser. Zool. 15, 131–135.

12.

12. Lock, L.F. and Soares, E.R. (1980). Increases in morphologically abnormal sperm in rats exposed to Co60 irradiation. Environ. Mutagen. 2(2). 125–131.

13.

13. Silvestrini, R., Di Marco, A. and Dasdia, T. (1970). Interference of dauonymcin with metabolic events of the cell cycle in synchronized cultures of rat fibroblast. 30, 966-973.

14.

14. Sternitt , and Hotta, Y. (1967). Chromosomal behaviour during development of meiotic tissue. In “The Control of Nuclear Activity,” L. Goldstein , Ed. Englewood Cliffs, N.J., Prentice-Hall, Inc.

Seminal Cytology and Reproductive Toxicology of N-Chloropiperidine

Abstract

References