Matrix metalloproteinase (MMP) is involved in the remodeling process of inflammatory airway diseases and is correlated with the severity of asthma. We hypothesized that MMP was associated with the severity of chronic rhinosinusitis with nasal polyps (CRSwNPs). We also investigated the effect of allergy on the expression of MMP in the polyp.
Methods
The expression of MMP-2 and -9 was investigated in recurrent nasal polyps of 30 patients and in nonrecurrent nasal polyps of 31 patients undergoing endoscopic sinus surgery. These expressions were then compared with those in control nasal mucosal samples obtained from 32 patients with chronic hypertrophic rhinitis. Demographic data, Lund-Mackay (LM) score, polyp grade, and allergy status were obtained for all patients. Tissue samples were assessed via immunohistochemistry.
Results
MMP-2 and -9 were constantly expressed in recurrent NPs, primary NPs, and control nasal mucosa. The expression of MMP-9 was significantly enhanced in glands and MMP-2 positivity was significantly increased in surface epithelium for patients with NPs when compared with control nasal mucosa. The expression of MMP-9 and -2 was not correlated with polyp grade and LM score. Allergic status is an independent factor in the expression of MMP-2 and -9.
Conclusion
These results suggested up-regulation of MMP-9, and MMP-2 in gland and surface epithelium, respectively, were characteristic of NPs. Therefore, patients with allergy will exhibit greater MMP-2 and -9 positivity. However, the MMP-2 and -9 expression intensity was not correlated with the severity of CRS with nasal polyposis.
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References
1.
PolzehlD., MoellerP., RiechelmannH.. Distinct features of chronic rhinosinusitis with and without nasal polyps.Allergy61: 1275–1279, 2006.
2.
BousquetJ., ChanezP., LacosteJ.Y.. Asthma: A disease remodeling the airways.Allergy47: 3–11, 1992.
3.
ShaidaA., KenyonG., DevaliaJ.. Matrix metalloproteinases and their inhibitors in the nasal mucosa of patients with perennial allergic rhinitis.J Allergy Clin Immunol108: 791–796, 2001.
4.
ShapiroS.D., and SeniorR.M.. Matrix metalloproteinases. Matrix degradation and more.Am J Respir Cell Mol Biol20: 1100–1102, 1999.
5.
Stetler-StevensonW.G.. Dynamics of matrix turnover during pathologic remodeling of the extracellular matrix.Am J Pathol148: 1345–1350, 1996.
6.
OkadaY., GonojiY., NakaK.. Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. Purification and activation of the precursor and enzymic properties.J Biol Chem267: 21712–21719, 1992.
7.
WelgusH.G., CampbellE.J., CuryJ.D.. Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development.J Clin Invest86: 1496–1502, 1990.
8.
HibbsM.S., HastyK.A., SeyerJ.M.. Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase.J Biol Chem260: 2493–2500, 1985.
9.
YaoP.M., BuhlerJ.M., d'OrthoM.P.. Expression of matrix metalloproteinase gelatinases A and B by cultured epithelial cells from human bronchial explants.J Biol Chem271: 15580–15589, 1996.
10.
WertS.E., YoshidaM., LeVineA.M.. Increased metalloproteinase activity, oxidant production, and emphysema in surfactant protein D gene-inactivated mice.Proc Natl Acad Sci U S A97: 5972–5977, 2000.
11.
HoshinoM., NakamuraY., SimJ.. Bronchial subepithelial fibrosis and expression of matrix metalloproteinase-9 in asthmatic airway inflammation.J Allergy Clin Immunol102: 783–788, 1998.
12.
BelleguicC., CorbelM., GermainN.. Increased release of matrix metalloproteinase-9 in the plasma of acute severe asthmatic patients.Clin Exp Allergy32: 217–223, 2002.
13.
ChenY., ChenP., HanaokaM.. Enhanced levels of prostaglandin E2 and matrix metalloproteinase-2 correlate with the severity of airflow limitation in stable COPD.Respirology13: 1014–1021, 2008.
14.
CundallM., SunY., MirandaC.. Neutrophil-derived matrix metalloproteinase-9 is increased in severe asthma and poorly inhibited by glucocorticoids.J Allergy Clin Immunol112: 1064–1071, 2003.
15.
WenzelS.E., BalzarS., CundallM.. Subepithelial basement membrane immunoreactivity for matrix metalloproteinase 9: Association with asthma severity, neutrophilic inflammation, and wound repair.J Allergy Clin Immunol111: 1345–1352, 2003.
16.
LeeY.M., KimS.S., KimH.A.. Eosinophil inflammation of nasal polyp tissue: Relationships with matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, and transforming growth factorbeta1.J Korean Med Sci18: 97–102, 2003.
17.
BhandariA., TakeuchiK., SuzukiS.. Increased expression of matrix metalloproteinase-2 in nasal polyps.Acta Otolaryngol124: 1165–1170, 2004.
18.
Lechapt-ZalcmanE., CosteA., d'OrthoM.P.. Increased expression of matrix metalloproteinase-9 in nasal polyps.J Pathol193: 233–241, 2001.
19.
WateletJ.B., BachertC., ClaeysC.. Matrix metalloproteinases MMP-7, MMP-9, and their tissue inhibitor TIMP-1: Expression in chronic sinusitis vs nasal polyposis.Allergy59: 54–60, 2004.
20.
WangJ.H., KwonH.J., and JangY.J.. Staphylococcus aureus increases cytokine and matrix metalloproteinase expression in nasal mucosae of patients with chronic rhinosinusitis and nasal polyps.Am J Rhinol Allergy24: 422–427, 2010.
21.
BugdayciG., KaymakciM., and BukanN.. Matrix metalloproteinase-9 (MMP-9) in allergic nasal polyps.Acta Histochem112: 92–95, 2010.
22.
WateletJ.B., DemetterP., ClaeysC.. Wound healing after paranasal sinus surgery: Neutrophilic inflammation influences the outcome.Histopathology48: 174–181, 2006.
WateletJ.B., ClaeysC., Van CauwenbergeP.. Predictive and monitoring value of matrix metalloproteinase-9 for healing quality after sinus surgery.Wound Repair Regen12: 412–418, 2004.
25.
FokkensW., LundV., and MullolJ.. EP3OS 2007: European position paper on rhinosinusitis and nasal polyps 2007. A summary for otorhinolaryngologists.Rhinology45: 97–101, 2007.
26.
LundV.J., and KennedyD.W.. Staging for rhinosinusitis.Otolaryngol Head Neck Surg117: S35–S40, 1997.
27.
LundV.J., and MackayI.S.. Staging in rhinosinusitis.Rhinology31: 183–184, 1993.
28.
Garcia-MarcosL., Sanchez-SolisM., Martinez-TorresA.E.. Phadiatop compared to skin-prick test as a tool for diagnosing atopy in epidemiological studies in schoolchildren.Pediatr Allergy Immunol18: 240–244, 2007.
29.
VidalC., GudeF., BoqueteO.. Evaluation of the Phadiatop test in the diagnosis of allergic sensitization in a general adult population.J Investig Allergol Clin Immunol15: 124–130, 2005.
30.
BuissonA.C., ZahmJ.M., PoletteM.. Gelatinase B is involved in the in vitro wound repair of human respiratory epithelium.J Cell Physiol166: 413–426, 1996.
31.
TournierJ.M., PoletteM., HinnraskyJ.. Expression of gelatinase A, a mediator of extracellular matrix remodeling, by tracheal gland serous cells in culture and in vivo.J Biol Chem269: 25454–25464, 1994.
32.
LelongtB., TrugnanG., MurphyG.. Matrix metalloproteinases MMP2 and MMP9 are produced in early stages of kidney morphogenesis but only MMP9 is required for renal organogenesis in vitro.J Cell Biol136: 1363–1373, 1997.
33.
ShimizuT., KanaiK., AsanoK.. Suppression of matrix metalloproteinase production in nasal fibroblasts by tranilast, an antiallergic agent, in vitro.Mediators Inflamm2005: 150–159, 2005.
34.
MattosW., LimS., RussellR.. Matrix metalloproteinase-9 expression in asthma: effect of asthma severity, allergen challenge, and inhaled corticosteroids.Chest122: 1543–1552, 2002.
35.
BoulayM.E., PrinceP., DeschesnesF.. Metalloproteinase-9 in induced sputum correlates with the severity of the late allergen-induced asthmatic response.Respiration71: 216–224, 2004.
36.
BaraldoS., BazzanE., ZaninM.E.. Matrix metalloproteinase-2 protein in lung periphery is related to COPD progression.Chest132: 1733–1740, 2007.
37.
DursunE., KorkmazH., EryilmazA.. Clinical predictors of long-term success after endoscopic sinus surgery.Otolaryngol Head Neck Surg129: 526–531, 2003.
38.
LiY., ZhangG.H., LiuX.. Clinical prognostic factors of chronic rhinosinusitis after endoscopic sinus surgery.ORL J Otorhinolaryngol Relat Spec70: 113–117, 2008.
