3 groups of 16 rats were trained to discriminate between two distinctive end boxes. Two groups discriminated between goal boxes while the third group discriminated between start boxes. These stimuli were then tested as secondary reinforcers in a T-maze. The groups trained to discriminate between goal boxes showed a statistically significant preference for the goal box previously associated with reinforcement. The third group showed no preference. It was concluded that discrimination training is not sufficient to establish an Sr without contiguity of stimulus and reward.
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