Corrigendum

Abstract

Oksvold MP, Skarpen E, Lindeman B, Roos N, Huitfeldt HS. Immunocytochemical localization of Shc and activated EGF receptor in early endosomes after EGF stimulation of HeLa cells. J Histochem Cytochem. 2000;48:21–33. (Original DOI: 10.1177/002215540004800103)

In this article, there was an error in the presentation of the data in Figure 4. Figure 4B incorrectly shows the anti-pY1173-EGFR micrograph used for Figure 6C, due to a mix-up of micrographs showing activated EGFR. The corrected image appears in the Figure 4A and 4B panel below (unstimulated and EGF-stimulated for 10 min, respectively).

Because the staining for EGFR and pY1173-EGFR in EGF-stimulated cells for 10 min is overlapping (e.g. Figure 6B in this paper), the error does not affect any conclusions in the published article. We apologize for any confusion the error may have caused.

Figure 4.

Immunocytochemical localization of EGFR in unstimulated cells (A), and activated EGFR in EGF-stimulated cells on ice without chase (C,E) and EGFR, and activated EGFR in EGF-stimulated cells chased for 10 min (B,D,F, respectively). Staining with anti-EGFR in unstimulated cells revealed a predominantly plasma membrane localization of total EGFR (A). Staining with R42/pY1173 (C) and R46/pY992 (E) in EGF-stimulated cells on ice without chase resulted in plasma membrane localization of activated EGFR. After a 10-min chase, EGFR, Y1173-, and Y992-phosphorylated EGFR were redistributed to vesicles throughout the cytoplasm (B,D,F, respectively). Bar = 10 μm.