Abstract
Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues. Monica Prasad, Qinglin Zhu, Yao Sun, Xiaofang Wang, Ashok Kulkarni, Adele Boskey, Jian Q. Feng, and Chunlin Qin. J Histochem Cytochem. 59(11):1009-1021. Original DOI: 10.1369/0022155411423406.
The authors have noted two errors in text on page 1010, Materials and Methods section, Isolation of RNA, RT-PCR and Real-time PCR subsection, paragraph one. A set of primer sequence and the RT-PCR product size were incorrectly entered:
“…The PCR amplification of the DSPP cDNA was then performed using a forward primer, 5′-TTGTTGAAAACTCTGTGGCTGTGC-3′, and a reverse primer, 5′-GGCATCGTTGAAAATGCGGAGGAA-3′.”
“…The RT-PCR product (510 bp) from the salivary glands was then sequenced (Northwoods DNA; Solway, MN).”
The correct primer sequence and RT-PCR product size are as follows:
The PCR amplification of the DSPP cDNA was then performed using a forward primer, 5′-CCAGTTAGTACCACTGGAAAGAGAC-3′, and a reverse primer, 5′-TCATGGTTGGTGCTATTCTTGATGC-3′.
The RT-PCR product (521 bp) from the salivary glands was then sequenced (Northwoods DNA; Solway, MN).