Raman and Raman optical activity (ROA) spectra are presented for adenosine and seven of its derivative ribonucleotides. Both of these spectroscopic techniques are shown to be sensitive to the site and degree of phosphorylation, with a considerable number of marker bands being identified for these ribonucleotides. ROA spectra are shown to provide the most sensitive diagnostic tool for phosphorylation characterization and quantification.
ZhangD.XieY.MrozekM. F.OrtizC.DavissonV. J., and Ben-AmotzD., Anal. Chem.75, 5703 (2003).
17.
KneippK.KneippH.KarthaV. B.ManoharanR.DeinumG.ItzkanI.DasariR. R., and FeldM. S., Phys. Rev. E: Stat. Phys.57, R6281 (1998).
18.
AtkinsP. W. and BarronL. D., Mol. Phys.16, 453 (1969).
19.
BarronL. D.BogaardM. P., and BuckinghamA. D., J. Am. Chem. Soc.95, 603 (1973).
20.
BarronL. D.HechtL.BlanchE. W., and BellA. F., Prog. Biophys. Mol. Biol.73, 1 (2000).
21.
AshtonL.JohannessenC., and GoodacreR., Anal. Chem.83, 20 (2011).
22.
LeeS.AndersonA.SmithW.GriffeyR. H., and MohanV., J. Raman Spectroscopy32, 795 (2001).
23.
RimaiL.ColeT.ParsonsJ. L.HickmottJ. T., and CarewE. B., Biophys. J.9, 320 (1969).
24.
SteinerR. F. and BeersR. F., Polynucleotides: Natural and Synthetic Nucleic Acids (Elsevier, Amsterdam, 1961), viii, pp. 305.
25.
LiaoY.MengY.LeiH., and WangY., Chin. Opt. Lett.6, 61 (2008).
26.
BellA. F.HechtL., and BarronL. D., J. Am. Chem. Soc.119, 6006 (1997).
27.
BellA. F.HechtL., and BarronL. D., J. Raman Spectrosc.30, 651 (1999).
28.
BellA. F.HechtL., and BarronL. D., Chem. Eur. J.3, 1292 (1997).
29.
BellA. F.HechtL., and BarronL. D., J. Chem. Soc., Faraday Trans.93, 553 (1997).
30.
BellA. F.HechtL., and BarronL. D., J. Am. Chem. Soc.120, 5820 (1998).
31.
BellA. F.HechtL., and BarronL. D., Biospectroscopy4, 107 (1998).
32.
FelsenfeldG.DaviesD. R., and RichA., J. Am. Chem. Soc.79, 2023 (1957).
33.
MathlouthiM.SeuvreA. M., and KoenigJ. L., Carbohydrate Res.131, 1 (1984).
34.
UedaT.UshizawaK., and TsuboiM., Spectrochimica Acta50, 1661 (1994).
35.
KoglinE.SequarisJ. M., and ValentaP., J. Mol. Struct.60, 421 (1980).
36.
HobroA. J., PhD thesis, Structural Investigation of RNA through the Application of Raman, Raman Optical Activity and Surface Enhanced Spectroscopies, Manchester, University of Manchester, UK (2008).